Metagenomics Approaches Based on Virion-Associated Nucleic Acids (VANA): An Innovative Tool for Assessing Without A Priori Viral Diversity of Plants

  • Denis Filloux
  • Sylvie Dallot
  • Agnès Delaunay
  • Serge Galzi
  • Emmanuel Jacquot
  • Philippe Roumagnac
Part of the Methods in Molecular Biology book series (MIMB, volume 1302)

Abstract

This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species.

Key words

Plant virus Virus discovery Diagnostic Dioscorea spp. Viral particles Random amplification Cloning 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Denis Filloux
    • 1
  • Sylvie Dallot
    • 2
  • Agnès Delaunay
    • 2
  • Serge Galzi
    • 1
  • Emmanuel Jacquot
    • 2
  • Philippe Roumagnac
    • 1
  1. 1.Cirad-INRA-Montpellier SupAgro, UMR BGPI, TA A-54/KCampus International de Montferrier-BaillarguetMontpellier Cedex 5France
  2. 2.INRA-Cirad-Montpellier SupAgro, UMR BGPI, TA A-54/KCampus International de Montferrier-BaillarguetMontpellier Cedex 5France

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