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Virus Testing by PCR and RT-PCR Amplification in Berry Fruit

  • Stuart MacFarlaneEmail author
  • Wendy McGavin
  • Ioannis Tzanetakis
Part of the Methods in Molecular Biology book series (MIMB, volume 1302)

Abstract

Berry fruit crops are prone to infection by a wide range of viruses, with the list expanding every year, primarily because of the expansion of the crops to new geographic regions. Although some methods allow for virus detection in a nonspecific manner, the advent of cheap and effective nucleic acid sequencing technologies has allowed for the development of species-specific tests. This chapter describes methods for extraction of nucleic acids for molecular testing from a range of different berry fruit crops and lists oligonucleotide primers that have been developed for amplification of a large number of berry fruit viruses.

Key words

Plant virus Berry fruit Strawberry Raspberry Blueberry Elderberry Detection RT-PCR RNA extraction DNA extraction 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Stuart MacFarlane
    • 1
    Email author
  • Wendy McGavin
    • 1
  • Ioannis Tzanetakis
    • 2
  1. 1.Cell and Molecular Sciences GroupThe James Hutton InstituteDundeeUK
  2. 2.University of ArkansasFayettevilleUSA

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