Measuring Ribonucleotide Incorporation into DNA In Vitro and In Vivo
Ribonucleotides are incorporated into genomes by DNA polymerases, they can be removed, and if not removed, they can have deleterious and beneficial consequences. Here, we describe an assay to quantify stable ribonucleotide incorporation by DNA polymerases in vitro, and an assay to probe for ribonucleotides in each of the two DNA strands of the yeast nuclear genome.
Key wordsRibonucleotide incorporation DNA replication DNA polymerase Ribonucleotide excision repair Alkali-sensitive sites
We thank Mercedes Arana and Katarzyna Bebenek for thoughtful comments on the manuscript and Kunkel lab members for technical expertise and discussions. This work was supported by Project Z01 ES065070 to T.A.K. from the Division of Intramural Research of the National Institutes of Health (NIH), National Institute of Environmental Health Sciences (NIEHS).
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