Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs
Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse-transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.
Key wordsmicroRNA Small non-coding RNA Real-time PCR quantification Stem-loop primers
This work was supported by the Centre National pour la Recherche Scientifique, the Université de Lorraine, the Région Lorraine and the Ligue nationale pour la recherche contre le cancer (comités 54 et 57).
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