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Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs

  • Véronique Salone
  • Mathieu RederstorffEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1296)

Abstract

Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse-transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.

Key words

microRNA Small non-coding RNA Real-time PCR quantification Stem-loop primers 

Notes

Acknowledgment

This work was supported by the Centre National pour la Recherche Scientifique, the Université de Lorraine, the Région Lorraine and the Ligue nationale pour la recherche contre le cancer (comités 54 et 57).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.CNRS UMR 7365, IMoPA, Université de LorraineVandoeuvre-lès-NancyFrance
  2. 2.Université de Lorraine, Biopôle, CNRS UMR 7365, IMoPAVandoeuvre-lès-NancyFrance

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