Analysis of Drug–Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin

  • Ryan Matsuda
  • Jeanethe Anguizola
  • Krina S. Hoy
  • David S. Hage
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1286)

Abstract

High-performance affinity chromatography (HPAC) is a type of liquid chromatography that has seen growing use as a tool for the study of drug–protein interactions. This report describes how HPAC can be used to provide information on the number of binding sites, equilibrium constants, and changes in binding that can occur during drug–protein interactions. This approach will be illustrated through recent data that have been obtained by HPAC for the binding of sulfonylurea drugs and other solutes to the protein human serum albumin (HSA), and especially to forms of this protein that have been modified by non-enzymatic glycation. The theory and use of both frontal analysis and zonal elution competition studies in such work will be discussed. Various practical aspects of these experiments will be presented, as well as factors to consider in the extension of these methods to other drugs and proteins or additional types of biological interactions.

Key words

Drug–protein binding High-performance affinity chromatography Biointeraction analysis Frontal analysis Zonal elution Sulfonylurea drugs Human serum albumin Glycation 
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Notes

Acknowledgements

This work was supported, in part, by the National Institutes of Health under grants R01 DK069629 and R01 GM044931. Additional support for R. Matsuda was obtained through a fellowship from the Molecular Mechanisms of Disease Program at the University of Nebraska-Lincoln.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Ryan Matsuda
    • 1
  • Jeanethe Anguizola
    • 1
  • Krina S. Hoy
    • 1
  • David S. Hage
    • 1
  1. 1.Department of ChemistryUniversity of Nebraska-LincolnLincolnUSA

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