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Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures

  • Hulda R. Jonsdottir
  • Ronald DijkmanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1282)

Abstract

The human airway serves as the entry point of human respiratory viruses, including human coronaviruses. In this chapter we outline the methods by which we establish fully differentiated airway epithelium and its use for human coronavirus propagation. Additionally, we outline methods for immunofluorescence staining of these cultures for virus detection, characterization of cell tropism, and how to perform antiviral assays and quantify viral replication.

Key words

Human coronavirus Antivirals Cell tropism Human airway epithelial cells Virus detection 

Notes

Acknowledgement

This work was supported by the 3R Research Foundation Switzerland (project 128-11).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Infectious Diseases and PathobiologyVetsuisse Faculty, University of BernBernSwitzerland
  2. 2.Federal Department of Home AffairsInstitute of Virology and ImmunologyBernSwitzerland

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