Coronaviruses pp 231-240 | Cite as

Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach

  • Jean Kaoru Millet
  • Béatrice NalEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1282)


Since its identification in the 1990s, the RNA interference (RNAi) pathway has proven extremely useful in elucidating the function of proteins in the context of cells and even whole organisms. In particular, this sequence-specific and powerful loss-of-function approach has greatly simplified the study of the role of host cell factors implicated in the life cycle of viruses. Here, we detail the RNAi method we have developed and used to specifically knock down the expression of ezrin, an actin binding protein that was identified by yeast two-hybrid screening to interact with the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) spike (S) protein. This method was used to study the role of ezrin, specifically during the entry stage of SARS-CoV infection.

Key words

RNA interference (RNAi) Small interfering RNA (siRNA) Ezrin Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Virus–host interactions 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Microbiology and ImmunologyCornell UniversityIthacaUSA
  2. 2.Department of Life Sciences, College of Health and Life SciencesBrunel UniversityLondonUK

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