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Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis

  • Stuart Dent
  • Benjamin W. NeumanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1282)

Abstract

Purification of intact enveloped virus particles can be useful as a first step in understanding the structure and function of both viral and host proteins that are incorporated into the virion. Purified preparations of virions can be used to address these questions using techniques such as mass spectrometry proteomics. Recent studies on the proteome of coronavirus virions have shown that in addition to the structural proteins, accessory and non-structural virus proteins and a wide variety of host cell proteins associate with virus particles. To further study the presence of virion proteins, high-quality sample preparation is crucial to ensure reproducible analysis by the wide variety of methods available for proteomic analysis.

Key words

Coronavirus Cryo-EM Proteomic Virus purification Density gradient centrifugation 

Notes

Acknowledgments

Funding for this work was provided by BBSRC and The Pirbright Institute.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.School of Biological SciencesUniversity of ReadingReadingUK

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