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NMR Spectroscopy of Biofluids and Extracts

  • Gwénaëlle Le GallEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1277)

Abstract

Metabonomics-based proton nuclear magnetic resonance (1H NMR) spectroscopy is a cross-disciplinary science that overlaps with analytical chemistry, biology, and statistical analysis. Applying 1H NMR on cell extracts provides a rapid and comprehensive screening of the most abundant metabolites allowing the quantitation of typically 20–70 compounds including amino and organic acids, sugars, amines, nucleosides, phenolic compounds, osmolytes, and lipids produced at sublevel millimolar concentrations. The method is particularly suited for high-throughput analysis (up to 100 samples/24 h), and the powerful structural elucidation of NMR is a great asset for the identification of unknown compounds. This chapter describes procedures for recording metabolite profiles using 1H NMR, depicts the preprocessing steps leading to data analysis, and presents methods of metabolite identification in spectral profiles of extracts from plants, food, microbes, and mammalian systems.

Key words

1H NMR Profiling Metabolomics Bucketing Urine Blood plasma Tissue Food extracts Microbial spent medium Mammalian cells 

Notes

Acknowledgment

This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Metabolomics UnitInstitute of Food ResearchNorwichUK

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