Methods to Study Tumor Surveillance Using Tumor Cell Transplantation into Genetically Engineered Mice

  • Eva Bauer
  • Agnieszka Witalisz
  • Birgit Strobl
  • Dagmar Stoiber
Part of the Methods in Molecular Biology book series (MIMB, volume 1267)


When a tumor evolves, there is constant crosstalk between the transformed cells and cells of the immune system. Transplantation of well-established tumor cell lines into genetically engineered mice is a valuable tool to study the contribution of a gene of interest to tumor surveillance. These methods bear several advantages: first, such cell lines are well characterized; second, much data for reference exist; and third, the impact of the immune system can be separated from tumor cell intrinsic effects. Here, we provide protocols for tumor cell transplantations to address the role of a specific gene product in tumor surveillance. We furthermore describe several approaches to define the impact of natural killer cells and T cells, such as cell depletion and adoptive transfer experiments or use of different genetically modified mice.

Key words

Tumor immune surveillance T cells Natural killer cells B16F10 EL4 EG7 MC38 RMA-S 



Financial support for this project was provided by the St. Anna Kinderkrebsforschung, Children’s Cancer Research Institute, financing the position of EB. BS is supported by the Austrian Science Fund (FWF) projects SFB-F28 and P25642-B22.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Eva Bauer
    • 1
  • Agnieszka Witalisz
    • 2
  • Birgit Strobl
    • 2
  • Dagmar Stoiber
    • 3
    • 4
  1. 1.Ludwig Boltzmann Institute for Cancer ResearchViennaAustria
  2. 2.Institute of Animal Breeding and GeneticsUniversity of Veterinary MedicineViennaAustria
  3. 3.Institute of PharmacologyMedical University of ViennaViennaAustria
  4. 4.Ludwig Boltzmann Institute for Cancer ResearchLudwig Boltzmann GesellschaftViennaAustria

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