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ETS and DEAS Studies of the Reduction of Xenobiotics in Mitochondrial Intermembrane Space

  • Stanislav A. PshenichnyukEmail author
  • Alberto Modelli
Part of the Methods in Molecular Biology book series (MIMB, volume 1265)

Abstract

This chapter describes the complementary experimental techniques electron transmission spectroscopy (ETS) and dissociative electron attachment spectroscopy (DEAS), two of the most suitable means for investigating interactions between electrons and gas-phase molecules, resonance formation of temporary molecular negative ions, and their possible decay through the dissociative electron attachment (DEA) mechanism. The latter can be seen as the gas-phase counterpart of the transfer of a solvated electron in solution, accompanied by dissociation of the molecular anion, referred to as dissociative electron transfer (DET). DET takes place in vivo under reductive conditions, for instance, in the intermembrane space of mitochondria under interaction of xenobiotic molecules with electrons “leaked” from the respiration chain. Experimental procedures supported by suitable quantum chemical calculations are described in detail and illustrated by an example of ETS/DEAS study of rhodanine which shows rich fragmentation under gas-phase resonance electron attachment.

Key words

Resonance electron attachment Temporary negative ions Xenobiotics One-electron reduction Molecular mechanism Mitochondria 

Notes

Acknowledgements

Thanks are due to the Russian Foundation for Basic Research (grants #12-03-00223-a and #14-03-00087-a), St. Petersburg State University (scientific project # 11.38.219.2014), and the Italian Ministero dell’Istruzione, dell’Università e della Ricerca for financial support.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Institute of Molecule and Crystal Physics, Ufa Research CentreRussian Academy of SciencesUfaRussia
  2. 2.Physics FacultySt. Petersburg State UniversitySt. PetersburgRussia
  3. 3.Dipartimento di Chimica “G. Ciamician”Università di BolognaBolognaItaly
  4. 4.Centro Interdipartimentale di Ricerca in Scienze AmbientaliRavennaItaly

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