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In Vivo Assessment of Mitochondrial Oxygen Consumption

  • Floor A. Harms
  • Egbert G. MikEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1264)

Abstract

The protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) has been proposed by us as a potential clinical noninvasive tool for monitoring mitochondrial function. We have been working on the development of mitochondrial respirometry for monitoring mitochondrial oxygen tension (mitoPO2) and mitochondrial oxygen consumption (mitoVO2) in skin. In this work we describe the principles of the method in experimental animals.

Key words

Oxygen-dependent quenching Mitochondrial oxygen tension (mitoPO2Mitochondrial oxygen consumption (mitoVO2Phosphorescence quenching Tissue oxygenation 

References

  1. 1.
    Ishii N (2007) Role of oxidative stress from mitochondria on aging and cancer. Cornea 26(9 Suppl 1):S3–S9PubMedCrossRefGoogle Scholar
  2. 2.
    Ballinger SW (2005) Mitochondrial dysfunction in cardiovascular disease. Free Radic Biol Med 38(10):1278–1295PubMedCrossRefGoogle Scholar
  3. 3.
    Fink MP (2002) Cytopathic hypoxia. Is oxygen use impaired in sepsis as a result of an acquired intrinsic derangement in cellular respiration? Crit Care Clin 18(1):165–175PubMedCrossRefGoogle Scholar
  4. 4.
    Galley HF (2011) Oxidative stress and mitochondrial dysfunction in sepsis. Br J Anaesth 107(1):57–64PubMedCrossRefGoogle Scholar
  5. 5.
    Garrabou G et al (2012) The effects of sepsis on mitochondria. J Infect Dis 205(3):392–400PubMedCrossRefGoogle Scholar
  6. 6.
    Edeas M, Weissig V (2014) Strategies, innovations & clinical applications. In: Edeas M, Weissig V (eds). Targeting mitochondria, BerlinGoogle Scholar
  7. 7.
    Mik EG et al (2006) Mitochondrial PO2 measured by delayed fluorescence of endogenous protoporphyrin IX. Nat Methods 3(11):939–945PubMedCrossRefGoogle Scholar
  8. 8.
    Mik EG et al (2009) Mitochondrial oxygen tension within the heart. J Mol Cell Cardiol 46(6):943–951PubMedCrossRefGoogle Scholar
  9. 9.
    Mik EG et al (2008) In vivo mitochondrial oxygen tension measured by a delayed fluorescence lifetime technique. Biophys J 95(8):3977–3990PubMedCentralPubMedCrossRefGoogle Scholar
  10. 10.
    Mik EG (2013) Measuring mitochondrial oxygen tension: from basic principles to application in humans. Anesth Analg 117(4):834–846PubMedCrossRefGoogle Scholar
  11. 11.
    Harms FA et al (2013) Cutaneous respirometry by dynamic measurement of mitochondrial oxygen tension for monitoring mitochondrial function in vivo. Mitochondrion 13(5):507–514PubMedCrossRefGoogle Scholar
  12. 12.
    Harms FA et al (2012) Validation of the protoporphyrin IX-triplet state lifetime technique for mitochondrial oxygen measurements in the skin. Opt Lett 37(13):2625–2627PubMedCrossRefGoogle Scholar
  13. 13.
    Golub AS, Tevald MA, Pittman RN (2011) Phosphorescence quenching microrespirometry of skeletal muscle in situ. Am J Physiol Heart Circ Physiol 300:H135–H143PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Anesthesiology, Laboratory of Experimental AnesthesiologyErasmus MC – University Medical Center RotterdamRotterdamThe Netherlands
  2. 2.Department of Intensive CareErasmus MC – University Medical Center RotterdamRotterdamThe Netherlands

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