In Vivo Assessment of Mitochondrial Oxygen Consumption

  • Floor A. Harms
  • Egbert G. MikEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1264)


The protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) has been proposed by us as a potential clinical noninvasive tool for monitoring mitochondrial function. We have been working on the development of mitochondrial respirometry for monitoring mitochondrial oxygen tension (mitoPO2) and mitochondrial oxygen consumption (mitoVO2) in skin. In this work we describe the principles of the method in experimental animals.

Key words

Oxygen-dependent quenching Mitochondrial oxygen tension (mitoPO2Mitochondrial oxygen consumption (mitoVO2Phosphorescence quenching Tissue oxygenation 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Anesthesiology, Laboratory of Experimental AnesthesiologyErasmus MC – University Medical Center RotterdamRotterdamThe Netherlands
  2. 2.Department of Intensive CareErasmus MC – University Medical Center RotterdamRotterdamThe Netherlands

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