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Production of Cell Surface and Secreted Glycoproteins in Mammalian Cells

  • Elena Seiradake
  • Yuguang ZhaoEmail author
  • Weixian Lu
  • A. Radu AricescuEmail author
  • E. Yvonne JonesEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1261)

Abstract

Mammalian protein expression systems are becoming increasingly popular for the production of eukaryotic secreted and cell surface proteins. Here we describe methods to produce recombinant proteins in adherent or suspension human embryonic kidney cell cultures, using transient transfection or stable cell lines. The protocols are easy to scale up and cost-efficient, making them suitable for protein crystallization projects and other applications that require high protein yields.

Key words

Protein expression HEK 293 cells Transient transfection Stable cell lines 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.The Division of Structural BiologyThe Henry Wellcome Building for Genomic MedicineOxfordUK

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