Dopamine Receptor Technologies pp 329-342

Part of the Neuromethods book series (NM, volume 96)

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Optogenetic Regulation of Dopamine Receptor-Expressing Neurons

Protocol

Abstract

Optogenetics has provided neuroscientists with the tools to control activity of specific neurons within a circuit. Optogenetic manipulation of dopamine receptor-containing neurons in the striatum holds great potential in understanding and treating a number of neuropsychiatric and neurological disorders. Coupling optogenetics with cell subtype-specific transgenic mouse lines permits dissection of dopamine receptor 1 (D1)- and dopamine receptor 2 (D2)-enriched circuits including the mesolimbic reward circuit and the basal ganglia circuit. This has led to multiple new insights into the function of dopamine receptor-expressing neurons in motivational and motor behaviors. This article discusses techniques to express microbial opsins in dopamine receptor-expressing neurons and to optogenetically activate or silence these neurons within the striatum in awake, behaving animals.

Key words

Optogenetics Dopamine receptors Channelrhodopsin Halorhodopsin Cre-inducible AAVs BAC transgenic animals Striatum Nucleus accumbens Medium spiny neurons 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Anatomy and NeurobiologyUniversity of Maryland School of MedicineBaltimoreUSA

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