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Genome-Wide Determination of Poly(A) Site Choice in Plants

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Polyadenylation in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1255))

Abstract

Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using high-throughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3′-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.

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Acknowledgements

This work was supported by US National Science Foundation (IOS-0817818) and the University of Kentucky Executive Vice President for Research. Liuyin Ma was a recipient of a scholarship from the China Scholarship Council, and Pratap Kumar Pati was supported by a Fulbright-Nehru Senior Research Fellowship.

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Author notes

    Authors and Affiliations

    1. Department of Biotechnology, Guru Nanak Dev University, Amritsar, India

      Pratap Kumar Pati

    2. Department of Horticulture, University of Kentucky, Lexington, KY, 40506, USA

      Pratap Kumar Pati

    3. Department of Plant and Soil Sciences, University of Kentucky, 105 Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40506, USA

      Liuyin Ma & Arthur G. Hunt

    4. Key Laboratory of Ministry of Education for Coastal Wetland Ecosystem, and College of the Environment and Ecology, Xiamen University, Fujian, 361102, China

      Liuyin Ma

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    1. Pratap Kumar Pati
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    2. Liuyin Ma
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    3. Arthur G. Hunt
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    Corresponding author

    Correspondence to Arthur G. Hunt .

    Editor information

    Editors and Affiliations

    1. Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, USA

      Arthur G. Hunt

    2. Department of Biology, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Miami University, Oxford, OH, USA and Xiamen University, Xiamen, Fujian, Fujian, China

      Qingshun Quinn Li

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    Pati, P.K., Ma, L., Hunt, A.G. (2015). Genome-Wide Determination of Poly(A) Site Choice in Plants. In: Hunt, A., Li, Q. (eds) Polyadenylation in Plants. Methods in Molecular Biology, vol 1255. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2175-1_14

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    • DOI: https://doi.org/10.1007/978-1-4939-2175-1_14

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-2174-4

    • Online ISBN: 978-1-4939-2175-1

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