Recombinant Expression and Purification of “Virus-like” Bacterial Encapsulin Protein Cages

  • W. Frederik Rurup
  • Jeroen J. L. M. Cornelissen
  • Melissa S. T. KoayEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1252)


Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of virus-like particles and protein cages. Since virus-like particles and protein cages have a unique size compared to cellular macromolecules and organelles, the rate of migration can be used as a tool for purification. Here we describe a detailed protocol for the purification of recently discovered virus-like assemblies called bacterial encapsulins from Thermotoga maritima and Brevibacterium linens.

Key words

Virus-like assemblies Protein cages Virus purification Bacterial encapsulins Nanotechnology 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • W. Frederik Rurup
    • 1
  • Jeroen J. L. M. Cornelissen
    • 1
  • Melissa S. T. Koay
    • 1
    • 2
    Email author
  1. 1.Laboratory for Biomolecular Nanotechnology, MESA + Institute for NanotechnologyUniversity of TwenteEnschedeThe Netherlands
  2. 2.Laboratory for Biomolecular Nanotechnology, MESA+ Institute for NanotechnologyUniversity of TwenteEnschedeThe Netherlands

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