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Evaluating the Spatial and Temporal Protein Production in Neural Tissue Engineering Constructs In Vitro

  • Caroline P. Addington
  • Christine Pauken
  • Sarah E. StabenfeldtEmail author
Protocol
Part of the Neuromethods book series (NM, volume 93)

Abstract

Tissue engineering constructs are uniquely positioned not only for in vivo regenerative therapeutic applications but also to evaluate cellular responses to modifications in finely tuned three-dimensional (3-D) microenvironments as opposed to traditional two-dimensional (2-D) culture systems. However, moving into a 3-D system presents complications of assessing spatial and temporal alterations in cellular behavior (e.g., protein synthesis, motility, etc.). Here, we describe methods to evaluate spatial and temporal protein production in neural tissue engineering construct.

Key words

Neural tissue engineering Western blot Immunocytochemistry Cryosectioning 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Caroline P. Addington
    • 1
  • Christine Pauken
    • 1
  • Sarah E. Stabenfeldt
    • 1
    Email author
  1. 1.School of Biological and Health Systems Engineering, Ira A. Fulton Schools of EngineeringArizona State UniversityTempeUSA

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