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General Cytotoxicity Assessment by Means of the MTT Assay

  • Laia Tolosa
  • María Teresa Donato
  • María José Gómez-LechónEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1250)

Abstract

Cytotoxicity assays were among the first in vitro bioassay methods used to predict toxicity of substances to various tissues. In vitro cytotoxicity testing provides a crucial means for safety assessment and screening, and for ranking compounds. The choice of using a particular cytotoxicity assay technology may be influenced by specific research goals. As such, four main classes of assays are used to monitor the response of cultured cells after treatment with potential toxicants. These methods measure viability, cell membrane integrity, cell proliferation, and metabolic activity. In this chapter, we focus on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium reduction colorimetric assay to evaluate detrimental intracellular effects on metabolic activity. This assay is well-characterized, simple to use and remains popular in several laboratories worldwide.

Key words

Cytotoxicity Tetrazolium salt IC50 Balb/c 3T3 cells Rat hepatocytes 

Notes

Acknowledgements

The authors acknowledge financial support from the ALIVE Foundation. Laia Tolosa was a recipient of a Sara Borrell Contract from the “Instituto de Salud Carlos III” of the Spanish Ministry of Economy and Competitiveness.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Laia Tolosa
    • 1
  • María Teresa Donato
    • 1
    • 2
    • 3
  • María José Gómez-Lechón
    • 1
    • 2
    Email author
  1. 1.Unidad de Hepatología ExperimentalInstituto de Investigación Sanitaria La FeValenciaSpain
  2. 2.CIBERehdFISBarcelonaSpain
  3. 3.Departamento de Bioquímica y Biología Molecular, Facultad de MedicinaUniversidad de ValenciaValenciaSpain

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