Impact of Next-Generation Technologies on Exploring Socioeconomically Important Parasites and Developing New Interventions

  • Cinzia Cantacessi
  • Andreas Hofmann
  • Bronwyn E. Campbell
  • Robin B. Gasser
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1247)

Abstract

High-throughput molecular and computer technologies have become instrumental for systems biological explorations of pathogens, including parasites. For instance, investigations of the transcriptomes of different developmental stages of parasitic nematodes give insights into gene expression, regulation and function in a parasite, which is a significant step to understanding their biology, as well as interactions with their host(s) and disease. This chapter (1) gives a background on some key parasitic nematodes of socioeconomic importance, (2) describes sequencing and bioinformatic technologies for large-scale studies of the transcriptomes and genomes of these parasites, (3) provides some recent examples of applications and (4) emphasizes the prospects of fundamental biological explorations of parasites using these technologies for the development of new interventions to combat parasitic diseases.

Key words

Parasitic nematodes Genomics Transcriptomics Bioinformatics Next-generation sequencing Post-genomics Anthelmintic resistance Drug targets Diagnostic markers 

Notes

Acknowledgments

Funding from the Australian Research Council, the National Health and Medical Research Council, the Australian Academy of Science, the Alexander von Humboldt Foundation, and Melbourne Water Corporation is gratefully acknowledged (RBG). Our research program was also supported by the Victorian Life Sciences Computation Initiative (grant number VR0007) on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government (RBG).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Cinzia Cantacessi
    • 1
    • 3
  • Andreas Hofmann
    • 2
  • Bronwyn E. Campbell
    • 1
  • Robin B. Gasser
    • 1
  1. 1.Department of Veterinary and Agricultural SciencesThe University of MelbourneParkvilleAustralia
  2. 2.Structural Chemistry Program, Eskitis InstituteGriffith UniversityBrisbaneAustralia
  3. 3.Department of Veterinary MedicineUniversity of CambridgeCambridgeUK

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