SNP Genotyping Using KASPar Assays

  • Scott M. Smith
  • Peter J. Maughan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1245)

Abstract

In a separate chapter we describe a simple method for single nucleotide polymorphism (SNP) discovery using genomic reduction. Here we describe a scalable and cost-effective SNP genotyping method based on KBioscience’s competitive allele-specific PCR amplification of target sequences and endpoint fluorescence genotyping (KASPar™) using a FRET capable plate reader or Fluidigm’s dynamic array high-throughput platform.

Key words

KASPar™ Single nucleotide polymorphism (SNP) Genotyping Fluidigm 

Notes

Acknowledgments

This research was funded by the McKnight Foundation and Ezra Taft Benson Agriculture and Food Institute. We gratefully acknowledge Dr. Joshua Udall (BYU), Jared Clouse (BYU) and Jamie Rice (Fluidigm) for his assistance and advice with regards to the Fluidigm protocols.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Scott M. Smith
    • 1
  • Peter J. Maughan
    • 2
  1. 1.Department of Plant and Microbial BiologyNorth Carolina State UniversityKannapolisNC
  2. 2.5144 LSB, Department of Plant and Wildlife SciencesBrigham Young UniversityProvoUSA

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