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Optimized Method for Growing In Vitro Arabidopsis thaliana Pollen Tubes

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Plant Cell Expansion

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1242))

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Abstract

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

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References

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Acknowledgement

This work was supported by PICT2011-0054 (J.M.E.) and PICT2011-1698 and PICT2012-0007 (J.M.).

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Authors and Affiliations

  1. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina

    Cecilia Borassi & José M. Estevez

  2. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Dr. Héctor Torres (INGEBI-CONICET), Vuelta de Obligado 2490, Buenos Aires, C1428ADN, Argentina

    Juliana Pérez Di Giorgio, María R. Scarpin & Jorge Muschietti

  3. Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina

    Jorge Muschietti

Authors
  1. Cecilia Borassi
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  2. Juliana Pérez Di Giorgio
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  3. María R. Scarpin
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  4. Jorge Muschietti
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  5. José M. Estevez
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Corresponding author

Correspondence to Jorge Muschietti .

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Editors and Affiliations

  1. Departamento de Fisiología Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina

    José M. Estevez

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Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M. (2015). Optimized Method for Growing In Vitro Arabidopsis thaliana Pollen Tubes. In: Estevez, J. (eds) Plant Cell Expansion. Methods in Molecular Biology, vol 1242. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1902-4_3

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  • DOI: https://doi.org/10.1007/978-1-4939-1902-4_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1901-7

  • Online ISBN: 978-1-4939-1902-4

  • eBook Packages: Springer Protocols

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