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“Multiplexed Viability, Cytotoxicity, and Caspase Activity Assays”

  • Andrew L. NilesEmail author
  • Terry L. Riss
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1219)

Abstract

Multiplexed assay chemistries provide for multiple measurements of cellular parameters within a single assay well. This experimental practice is not only more cost efficient, but also provides more information about a compound or treatment. The ability to combine the activity profiles within the same sample provides a level of normalization not possible with parallel assays. Furthermore, multiplexing caspase activity assays with viability and/or cytotoxicity assays can support conclusions regarding cytotoxic mechanism and provide normalization, which may help correct for differences in cell number.

Key words

Multiplex Fluorescence Luminescence Cell-based Cytotoxicity Caspase 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Promega CorporationMadisonUSA

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