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Gene Expression Analysis in Early Embryos Through Reverse Transcription Quantitative PCR (RT-qPCR)

  • Nathalie Peynot
  • Véronique Duranthon
  • Daulat Raheem KhanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1222)

Abstract

Real-time, reverse transcription quantitative PCR (RT-qPCR) is a highly sensitive and reproducible technology for the analysis of gene expression patterns. Its ability to detect minute quantities of nucleic acid from multifarious sources makes it an ideal technique for embryonic transcript quantification. However, complex cellular diversity and active transcriptome dynamics in early embryos necessitate particular caution to avoid erroneous results. This chapter is intended to outline basic methodology to design and execute RT-qPCR experiments in pre-implantation embryos.

Key words

Embryo Gene expression Quantitative-PCR RT-qPCR Data analysis Reference genes 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Nathalie Peynot
    • 1
  • Véronique Duranthon
    • 1
  • Daulat Raheem Khan
    • 2
    Email author
  1. 1.UMR1198 Biologie du Développement et ReproductionINRAJouy-en-JosasFrance
  2. 2.Centre de Recherche en Biologie de la Reproduction (CRBR)Université LavalQuébecCanada

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