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Magnetic Bead-Based Isolation of Exosomes

  • Morten P. Oksvold
  • Axl Neurauter
  • Ketil W. PedersenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1218)

Abstract

Exosomes are here defined as extracellular vesicles (EVs) in the approximate size range of 30–100 nm in diameter, and are observed in most body fluids containing typical exosomal markers such as CD9, CD63, and CD81. Potential subpopulations of exosomes can be captured by targeting these markers using magnetic beads. Magnetic beads are versatile tools for exosome isolation and downstream analysis. Here, we describe the workflow of immuno magnetic isolation and analysis of exosomes by flow cytometry, Western immunoblotting, and electron microscopy.

Key words

Extracellular vesicles Exosomes Magnetic bead isolation Dynabeads Electron microscopy 

Notes

Acknowledgement

Bente Kierulf, Anette Kullman, and Malin Karlsson (Life Technologies) for expert advice and critically reading the manuscript, Sasha Vlassov and Mu Li (Life Technologies) for NanoSight® data, Antje Hoenen and Norbert Roos (University of Oslo, Norway) for technical assistance in electron microscopy.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Morten P. Oksvold
    • 1
    • 2
  • Axl Neurauter
    • 3
  • Ketil W. Pedersen
    • 3
    Email author
  1. 1.Department of Immunology, Institute for Cancer ResearchOslo University HospitalOsloNorway
  2. 2.Centre for Cancer BiomedicineUniversity of OsloOsloNorway
  3. 3.Life Technologies ASSmestadNorway

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