Probing Protein Targeting to Plasmodesmata Using Fluorescence Recovery After Photo-Bleaching
Fluorescence recovery after photo-bleaching (FRAP) involves the irreversible bleaching of a fluorescent protein within a specific area of the cell using a high-intensity laser. The recovery of fluorescence represents the movement of new protein into this area and can therefore be used to investigate factors involved in this movement. Here we describe a FRAP method to investigate the effect of a range of pharmacological agents on the targeting of Tobacco mosaic virus movement protein to plasmodesmata.
Key wordsTobacco mosaic virus Movement protein Plasmodesmata Fluorescence recovery after photo-bleaching
This work was funded by the Scottish Government Rural and Environmental Science and Analytical Services Division (RESAS). We thank D.A. Elston and P.J. Wright for critical review and helpful comments.
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