Automatic Flow Injection Analysis (FIA) Determination of Total Reducing Capacity in Serum and Urine Samples

  • Marcela A. SegundoEmail author
  • Ildikó V. Tóth
  • Luís M. Magalhães
  • Salette Reis
Part of the Methods in Molecular Biology book series (MIMB, volume 1208)


Automation of total antioxidant capacity assessment can substantially increase the determination throughput, allowing large scale studies and screening experiments. Total reducing capacity evaluation can be implemented under different chemistries, including the CUPRAC—Cupric Ion Reducing Antioxidant Capacity —assay. This assay is based on reduction of Cu(II)-neocuproine complex to highly colored Cu(I)-neocuproine complex by reducing (antioxidant) components of biological samples. In this chapter, we propose an automatic flow injection method for evaluation of total reducing capacity in serum and urine samples, attaining end-point data within 4 min using a kinetic matching strategy.

Key words

Total reducing capacity CUPRAC assay Flow injection analysis Biological samples Automation 



L.M. Magalhães and I.V. Tóth thank FSE (Fundo Social Europeu) and MCTES (Ministério da Ciência, Tecnologia e Ensino Superior) for the financial support through the POPH-QREN program. The authors also acknowledge to the Fundação para a Ciência e a Tecnologia for the financial support through Strategic Project PEst-C/EQB/LA0006/2013.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Marcela A. Segundo
    • 1
    Email author
  • Ildikó V. Tóth
    • 1
  • Luís M. Magalhães
    • 1
  • Salette Reis
    • 1
  1. 1.REQUIMTE, Department of Chemical Sciences, Faculty of PharmacyUniversity of PortoPortoPortugal

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