Plant Endosomes pp 265-283

Part of the Methods in Molecular Biology book series (MIMB, volume 1209)

| Cite as

Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis

  • Tomáš Takáč
  • Tibor Pechan
  • Olga Šamajová
  • Jozef Šamaj
Protocol

Abstract

We present a comprehensive approach combining proteomics and cell biology to study vesicular trafficking in plants. Within this approach, we exploit chemical compounds inhibiting particular vesicular trafficking events in plant cells. Treatment of plants with these relatively specific inhibitors results in intracellular accumulation of proteins being transported by vesicles as well as in a change in abundance of regulatory proteins. Such pharmacological inhibition allows for identification of key proteins, and for further detailed functional investigation using cell biological, molecular biological, and biochemical methods used for validation of proteomic results.

Key words

Endocytosis Vesicular trafficking Proteomics Plants 2-D LC/MSMS 2-D electrophoresis Inhibitors Brefeldin A Wortmannin LY294002 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Tomáš Takáč
    • 1
  • Tibor Pechan
    • 2
  • Olga Šamajová
    • 1
  • Jozef Šamaj
    • 1
  1. 1.Department of Cell Biology, Faculty of Science, Centre of the Region Haná for Biotechnological and Agricultural ResearchPalacký University OlomoucOlomoucCzech Republic
  2. 2.Institute for Genomics, Biocomputing and BiotechnologyMississippi State UniversityMississipi StateUSA

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