RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells

  • Vidisha Tripathi
  • Jingyi Fei
  • Taekjip Ha
  • Kannanganattu V. PrasanthEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1206)


It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.

Key words

Long noncoding RNA lncRNA In situ hybridization RNA-FISH Fluorescence 



Research in the KVP lab is supported by grants from NIH/NIGMS (GM088252) and American Cancer Society (RSG-11-174-01-RMC). TH is an HHMI investigator.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Vidisha Tripathi
    • 1
  • Jingyi Fei
    • 2
  • Taekjip Ha
    • 3
  • Kannanganattu V. Prasanth
    • 1
    Email author
  1. 1.Department of Cell and Developmental BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Department of Physics, Center of the Physics of Living CellsUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  3. 3.Center for the Physics of Living Cells, Department of Physics, Center for Biophysics and Computational Biology, Institute for Genomic BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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