Nanoparticle-Assisted Laser Desorption/Ionization for Metabolite Imaging

  • Michihiko Waki
  • Eiji Sugiyama
  • Takeshi Kondo
  • Keigo Sano
  • Mitsutoshi SetouEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1203)


Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) has enabled the spatial analysis of various molecules, including peptides, nucleic acids, lipids, and drug molecules. To expand the capabilities of MALDI-IMS, we have established an imaging technique using metal nanoparticles (NPs) to visualize metabolites, termed nanoparticle-assisted laser desorption/ionization imaging mass spectrometry (nano-PALDI-IMS). By utilizing Ag-, Fe-, Au-, and TiO2-derived NPs, we have succeeded in visualizing various metabolites, including fatty acid and glycosphingolipids, with higher sensitivity and spatial resolution than conventional techniques. Herein, we describe the practical experimental procedures and methods associated with nano-PALDI-IMS for the visualization of these molecules.

Key words

Imaging mass spectrometry Nanoparticles Matrix Fatty acids Glycosphingolipids Metabolites 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Michihiko Waki
    • 1
  • Eiji Sugiyama
    • 1
  • Takeshi Kondo
    • 1
  • Keigo Sano
    • 1
  • Mitsutoshi Setou
    • 1
    Email author
  1. 1.Department of Cell Biology and AnatomyHamamatsu University School of MedicineHamamatsu-shiJapan

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