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Lectins pp 47-52 | Cite as

Hemagglutination (Inhibition) Assay

  • Kotone Sano
  • Haruko OgawaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1200)

Abstract

The hemagglutination assay is a simple and easy method to obtain semi-quantitative data on the sugar binding and specificity of a lectin. An active lectin agglutinates erythrocytes by recognizing a carbohydrate on the cell surface and forming a cross-linked network in suspension. By serially diluting the lectin in a 96-well microtiter plate and adding a constant quantity of erythrocytes, the lectin activity can be estimated.

Key words

Hemagglutination Hemagglutinin Agglutinin Carbohydrate recognition Erythrocyte 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Graduate School of Humanities and Sciences and Glycoscience InstituteOchanomizu UniversityBunkyo-kuJapan

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