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Quantum Dot–Antibody Conjugates via Carbodiimide-Mediated Coupling for Cellular Imaging

  • Daniel Alistair East
  • Michael Todd
  • Ian James BruceEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1199)

Abstract

This chapter describes the processes of antibody (Ab) production, purification, conjugation to quantum dots (QDs), and the use of the conjugates produced in intracellular imaging of cell components and structures. Specifically, information is provided on the conjugation of carboxyl surface-terminated QDs to Abs via a one-step reaction using the water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). The chapter details the process of conjugate optimization in terms of its final fluorescence and biological activity. The method described should guarantee the production of QD–Ab conjugates, which outperform classic organic fluorophore–Ab conjugates in terms of both image definition produced and the longevity of the imaging agent.

Key words

Quantum dot Antibodies Conjugation Conjugate Carbodiimide EDC Fluorescence Imaging 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Daniel Alistair East
    • 1
  • Michael Todd
    • 2
  • Ian James Bruce
    • 3
    Email author
  1. 1.Royal Veterinary CollegeLondonUK
  2. 2.School of ScienceUniversity of GreenwichChatham Maritime KentUK
  3. 3.School of Physical SciencesUniversity of KentCanterburyUK

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