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Drosophila as a Model for Intestinal Infections

  • Matthieu Lestradet
  • Kwang-Zin Lee
  • Dominique Ferrandon
Part of the Methods in Molecular Biology book series (MIMB, volume 1197)

Abstract

Drosophila melanogaster is a powerful model to study infections thanks to the power of its genetics and knowledge on its biology accumulated for over a century. While the systemic humoral immune response against invading microbes has been intensively studied in the past two decades, the study of intestinal infections is more recent. Here, we present the methods that are currently in use to probe various aspects of the host-pathogen interactions between Drosophila and ingested microbes, with an emphasis on the study of the midgut epithelium, which constitutes the major interface between the organism and the microbe-rich ingested food.

Key words

Drosophila melanogaster Innate immunity Host-pathogen interactions Resilience/tolerance Intestinal stem cells Enterocyte Phagocytosis Serratia marcescens Pseudomonas aeruginosa Intestinal homeostasis 

Notes

Acknowledgements

We acknowledge the expert technical help from Mme Marie-Céline Lacombe and thank Mme Samantha Haller for advice. Work in the author’s laboratory has been funded by CNRS, ANR (DROSELEGANS, DROSOGUT, KANJI), Fondation Simone et Cino Del Duca, and Fondation pour la Recherche Médicale (Equipe FRM).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Matthieu Lestradet
    • 1
  • Kwang-Zin Lee
    • 1
  • Dominique Ferrandon
    • 1
  1. 1.UPR9022 du CNRS, Université de StrasbourgStrasbourg CedexFrance

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