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Hox Genes pp 49-57 | Cite as

A Genetic Strategy to Obtain P-Gal4 Elements in the Drosophila Hox Genes

  • Luis de Navas
  • David Foronda
  • Delia del Saz
  • Ernesto Sánchez-HerreroEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1196)

Abstract

The Drosophila Gal4/UAS system allows the expression of any gene of interest in restricted domains. We devised a genetic strategy, based on the P-element replacement and UAS-y + techniques, to generate Gal4 lines inserted in Hox genes of Drosophila that are, at the same time, mutant for the resident genes. This makes possible to express different wild-type or mutant Hox proteins in the precise domains of Hox gene expression, and thus to test the functional value of these proteins in mutant rescue experiments.

Key words

Drosophila Gal4/UAS system P-element Ultrabithorax Abdominal-B 

Notes

Acknowledgements

Work in the laboratory is being supported by a grant from the Spanish Ministerio de Economía y Competitividad (BFU2011-26075) and an institutional grant from the Fundación Ramón Areces. Delia del Saz is being supported by an FPI fellowship from the Spanish Ministerio de Economía y Competitividad.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Luis de Navas
    • 1
  • David Foronda
    • 1
    • 2
  • Delia del Saz
    • 1
  • Ernesto Sánchez-Herrero
    • 1
    Email author
  1. 1.Centro de Biología Molecular Severo Ochoa (CSIC-UAM)Universidad Autónoma de MadridMadridSpain
  2. 2.Institute of Molecular and Cell BiologySingaporeSingapore

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