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Hox Genes pp 307-318 | Cite as

Bimolecular Fluorescence Complementation (BiFC) in Live Drosophila Embryos

  • Marilyne Duffraisse
  • Bruno Hudry
  • Samir MerabetEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1196)

Abstract

Bimolecular fluorescence complementation (BiFC) is a powerful method for studying protein-protein interactions in different cell types and organisms. This method was recently developed in the fruit fly Drosophila melanogaster, allowing analyzing protein interaction properties in a physiologically relevant developing context. Here we present a detailed protocol for performing BiFC with the Venus fluorescent protein in live Drosophila embryos, taking the Hox-PBC partnership as an illustrative test case. This protocol applies to any transcription factor and split fluorescent protein in general.

Key words

Hox Transcription BiFC EMSA Drosophila 

Notes

Acknowledgement

This work was supported by the ARC (Association pour la Recherche sur le Cancer), and FRM (Fondation pour la Recherche Médicale).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Marilyne Duffraisse
    • 1
  • Bruno Hudry
    • 2
  • Samir Merabet
    • 1
    Email author
  1. 1.Institut de Génomique Fonctionnelle de LyonENS de Lyon - CNRS UMR5242Lyon Cedex 07France
  2. 2.MRC Clinical Sciences CentreImperial College LondonLondonUK

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