Bimolecular Fluorescence Complementation (BiFC) in Live Drosophila Embryos
Bimolecular fluorescence complementation (BiFC) is a powerful method for studying protein-protein interactions in different cell types and organisms. This method was recently developed in the fruit fly Drosophila melanogaster, allowing analyzing protein interaction properties in a physiologically relevant developing context. Here we present a detailed protocol for performing BiFC with the Venus fluorescent protein in live Drosophila embryos, taking the Hox-PBC partnership as an illustrative test case. This protocol applies to any transcription factor and split fluorescent protein in general.
Key wordsHox Transcription BiFC EMSA Drosophila
This work was supported by the ARC (Association pour la Recherche sur le Cancer), and FRM (Fondation pour la Recherche Médicale).