Hox Genes pp 197-208 | Cite as

Hox Transcriptomics in Drosophila Embryos

  • Maria Polychronidou
  • Ingrid LohmannEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1196)


Hox proteins are evolutionarily conserved homeodomain containing transcription factors that specify segment identities along the anteroposterior axis of almost all bilaterian animals. They exert their morphogenetic role by transcriptionally regulating a large battery of downstream target genes. Therefore the dissection of transcriptional networks regulated by Hox proteins is an essential step towards a mechanistic understanding of how these transcription factors coordinate multiple developmental and morphogenetic processes. High-throughput techniques allowing whole-transcriptome mRNA expression profiling are powerful tools for the genome-wide identification of Hox downstream target genes in a variety of experimental settings. Here, we describe how to quantitatively identify Hox downstream genes in Drosophila embryos by performing a Hox transcriptome analysis using microarrays.

Key words

Hox Drosophila Transcription Microarray Transcriptome Expression profiling 



We would like to thank Jan U. Lohmann and Markus Schmid for sharing protocols with us.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Centre for Organismal Studies (COS) HeidelbergUniversity of HeidelbergHeidelbergGermany

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