T-Helper Cells pp 171-185 | Cite as

Aldara-Induced Psoriasis-Like Skin Inflammation: Isolation and Characterization of Cutaneous Dendritic Cells and Innate Lymphocytes

  • C. T. Wohn
  • S. Pantelyushin
  • J. L. Ober-Blöbaum
  • B. E. Clausen
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1193)

Abstract

Psoriasis is a chronic auto-inflammatory skin disease of unknown etiology affecting millions of people worldwide. Dissecting the cellular networks and molecular signals promoting the development of psoriasis critically depends on appropriate animal models. Topical application of Aldara cream containing the Toll-like receptor (TLR)7-ligand Imiquimod induces skin inflammation and pathology in mice closely resembling plaque-type psoriasis in humans. The particular power of the Aldara model lies in examining the early events during psoriatic plaque formation, which is difficult to achieve in patients. Hence, recent reports using this model have challenged currently prevailing concepts concerning the pathophysiology of psoriasis. Here, we describe the induction and phenotype of Aldara-mediated dermatitis in mice and, in particular, analysis of the inflammatory cell infiltrate using flow cytometry.

Key words

Aldara cream Dendritic cells γδ T cells Imiquimod Innate lymphocytes Psoriasis Psoriatic skin inflammation 
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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • C. T. Wohn
    • 1
  • S. Pantelyushin
    • 2
  • J. L. Ober-Blöbaum
    • 3
  • B. E. Clausen
    • 3
  1. 1.Department of Immunology, Erasmus MCUniversity Medical Center RotterdamRotterdamThe Netherlands
  2. 2.Institute of Experimental ImmunologyUniversity of ZürichZürichSwitzerland
  3. 3.Institute for Molecular MedicineUniversity Medical Center of the Johannes Gutenberg-University MainzMainzGermany

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