Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1192)

Abstract

Cultured human mast cells are a useful tool for research into innate immune responses as well as allergic mechanisms. Mast cells cultured from peripheral blood can provide information on immune mechanisms of known, selected individuals. With the method presented here eight million mast cells can be cultured from ca. one million stem cells purified from one unit (450 mL) of human peripheral blood. Culture with IgE and IL4 optimizes an allergic phenotype of the mast cells.

Key words

Mast cell Human stem cells Buffy coat Cultured mast cells SCF CD63 FcεRI 
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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Clinical MedicineUniversity of AarhusAarhus NDenmark
  2. 2.The Department of Pulmonary MedicineAarhus University HospitalAarhus CDenmark

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