Identification of Murine Basophils by Flow Cytometry and Histology

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1192)

Abstract

Here we describe how murine basophils can be detected in vivo by flow cytometry and immunofluorescence staining. Basophils constitute a homogeneous population of CD4−CD19−CD49b+IgE+ cells in flow cytometric analysis. When IgE levels are low one can also use anti-FcεRI or anti-CD200R3 antibodies instead of anti-IgE. For immunofluorescence staining we use an anti-Mcpt8 antibody since Mcpt8 is a specific marker for murine basophils. We describe how to prepare the tissue to cut cryo-sections and how to perform the staining using a tyramide-based amplification kit.

Key words

Basophils Mcpt8 Flow cytometry Immunofluorescence staining Tyramide amplification 
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Notes

Acknowledgements

This work was supported in part by an ERC starting grant (PAS_241506) from the European Union.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Infection BiologyUniversity Clinic Erlangen and Friedrich-Alexander-University Erlangen-Nuremberg (FAU)ErlangenGermany

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