Use of Humanized Rat Basophil Leukemia (RBL) Reporter Systems for Detection of Allergen-Specific IgE Sensitization in Human Serum

  • Daniel Wan
  • Xiaowei Wang
  • Ryosuke Nakamura
  • Marcos J. C. Alcocer
  • Franco H. Falcone
Part of the Methods in Molecular Biology book series (MIMB, volume 1192)

Abstract

Determination of allergen-specific IgE levels in human blood samples is an important diagnostic technology for assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines which offer higher sensitivity and are amenable to high-throughput-scale experiments.

Key words

Reporter system Luciferase DsRed RBL IgE Allergy RS-ATL8 NFAT-DsRed 

Notes

Acknowledgements

This work was supported by the COST Action BM1007 Mast Cells and Basophils—Targets for Innovative Therapies.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Daniel Wan
    • 1
  • Xiaowei Wang
    • 1
  • Ryosuke Nakamura
    • 3
  • Marcos J. C. Alcocer
    • 1
  • Franco H. Falcone
    • 2
  1. 1.School of Pharmacy, The University of NottinghamNottinghamUK
  2. 2.Division of Molecular and Cellular Science, The School of PharmacyThe University of NottinghamNottinghamUK
  3. 3.National Institute of Health SciencesTokyoJapan

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