Tissue Morphogenesis pp 163-169

Part of the Methods in Molecular Biology book series (MIMB, volume 1189)

Culture of Mouse Embryonic Foregut Explants

Protocol

Abstract

The ability to culture embryonic organ rudiments and follow their development ex vivo has helped to understand how tissues are constructed and what cellular and biological events are important in this process. Here we outline a technique for isolation and ex vivo growth of foregut explants from E8.5 mouse embryos. This technique serves as a reliable tool for the analysis of the morphogenetic processes and signaling networks during early development of foregut derivatives, such as the lungs.

Key words

Organ explant Foregut culture Development Organogenesis Mouse Lung development 

References

  1. 1.
    Lanza RP, Langer R, Vacanti J (2000) Principles of tissue engineering, 2nd edn. Academic, San Diego, CAGoogle Scholar
  2. 2.
    Barak H, Boyle SC (2011) Organ culture and immunostaining of mouse embryonic kidneys. Cold Spring Harb Protoc. doi:10.1101/pdb.prot5558 PubMedGoogle Scholar
  3. 3.
    Taderera JV (1967) Control of lung differentiation in vitro. Dev Biol 16(5):489–512PubMedCrossRefGoogle Scholar
  4. 4.
    Kratochwil K (1969) Organ specificity in mesenchymal induction demonstrated in the embryonic development of the mammary gland of the mouse. Dev Biol 20(1):46–71, PMID: 5795848PubMedCrossRefGoogle Scholar
  5. 5.
    Wessells NK, Cohen JH (1967) Early pancreas organogenesis. Morphogenesis, tissue interactions, and mass effects. Dev Biol 15:237–270PubMedCrossRefGoogle Scholar
  6. 6.
    Spooner BS, Wessells NK (1970) Mammalian lung development: interactions in primordium formation and bronchial morphogenesis. J Exp Zool 175(4):445–454, PMID: 5501462PubMedCrossRefGoogle Scholar
  7. 7.
    Wessells NK (1970) Mammalian lung development: interactions in formation and morphogenesis of tracheal buds. J Exp Zool 175(4):455–466, PMID: 5501463PubMedCrossRefGoogle Scholar
  8. 8.
    Zorn AM, Wells JM (2009) Vertebrate endoderm development and organ formation. Annu Rev Cell Dev Biol 25:221–251, PMID: 19575677PubMedCrossRefPubMedCentralGoogle Scholar
  9. 9.
    Cardoso WV, Lu J (2006) Regulation of early lung morphogenesis: questions, facts and controversies. Development 133(9):1611–1624PubMedCrossRefGoogle Scholar
  10. 10.
    Morrisey EE, Hogan BL (2010) Preparing for the first breath: genetic and cellular mechanisms in lung development. Dev Cell 18(1):8–23PubMedCrossRefPubMedCentralGoogle Scholar
  11. 11.
    Desai TJ, Malpel S, Flentke GR et al (2004) Retinoic acid selectively regulates Fgf10 expression and maintains cell identity in the prospective lung field of the developing foregut. Dev Biol 273:402–415PubMedCrossRefGoogle Scholar
  12. 12.
    Desai TJ, Chen F, Lu J et al (2006) Distinct roles for retinoic acid receptors alpha and beta in early lung morphogenesis. Dev Biol 291:12–24PubMedCrossRefGoogle Scholar
  13. 13.
    Chen F, Desai TJ, Qian J et al (2007) Inhibition of Tgf beta signaling by endogenous retinoic acid is essential for primary lung bud formation. Development 134:2969–2979PubMedCrossRefGoogle Scholar
  14. 14.
    Chen F, Cao Y, Qian J et al (2010) A retinoic acid-dependent network in the foregut controls formation of the mouse lung primordium. J Clin Invest 120:2040–2048PubMedCrossRefPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Pulmonary Center – Boston University School of MedicineBostonUSA
  2. 2.Department of MedicineColumbia University Medical Center and Columbia Center for Human DevelopmentNew YorkUSA

Personalised recommendations