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Characterization and Activity of Fas Ligand Producing CD5+ B Cells

  • Steven K. Lundy
  • Matthew W. Klinker
Part of the Methods in Molecular Biology book series (MIMB, volume 1190)

Abstract

B lymphocytes make several contributions to immune regulation including production of antibodies with regulatory properties, release of immune suppressive cytokines, and expression of death-inducing ligands. A role for Fas ligand (FasL)-expressing “killer” B cells in regulating T helper cell survival and chronic inflammation has been demonstrated in animal models of schistosome worm infection, asthma, and autoimmune arthritis. Interestingly, a population of CD5+ B cells found in the spleen and lungs of naïve mice constitutively expresses FasL and has potent killer function against T helper cells that is antigen-specific and FasL-dependent. Killer B cells therefore represent a novel target for immune modulation in many disease settings. Our laboratory has recently published methods of characterizing FasL+ B cells and inducing their proliferation in vitro. This chapter will describe detailed methods of identifying and expanding killer B cells from mice, detecting FasL expression in B cells, and performing functional killing assays against antigen-specific TH cells.

Key words

Killer B lymphocytes T cell apoptosis Antigen-specific tolerance Death ligands IL-10 Immune suppression IL-5 CD40 ligand Lethal exosomes 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Division of Rheumatology, Department of Internal MedicineUniversity of Michigan Medical SchoolAnn ArborUSA
  2. 2.Immunology Training Program, Program in Biomedical Sciences, Rackham Graduate SchoolUniversity of MichiganAnn ArborUSA

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