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Visualizing Notch Signaling In Vivo in Drosophila Tissues

  • Benjamin E. Housden
  • Jinghua Li
  • Sarah J. BrayEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1187)

Abstract

The ability to visualize Notch pathway activity in vivo is invaluable for studying the functions and mechanisms of Notch signaling. A variety of tools have been developed to enable monitoring of pathway activity in Drosophila, including endogenous Notch-responsive genes and synthetic transcriptional reporter constructs. Here we summarize some of the different Notch signaling reporters that are available, discuss their relative merits, and describe two methods for visualizing their expression (immunostaining and X-gal staining). These approaches are widely applicable to a range of tissues and stages in Drosophila development.

Key words

Notch signaling Notch reporters Transcriptional reporters Antibodies X-gal staining Immunostaining Fluorescent reporters 

Notes

Acknowledgements

We would like to thank Hamid Moosavi for contributing data used in Fig. 1.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Benjamin E. Housden
    • 1
    • 2
  • Jinghua Li
    • 2
  • Sarah J. Bray
    • 2
    Email author
  1. 1.Department of GeneticsHarvard Medical SchoolBostonUSA
  2. 2.Department of Physiology, Development and NeuroscienceUniversity of CambridgeCambridgeUK

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