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RNA Mapping pp 289-305 | Cite as

miRWalk Database for miRNA–Target Interactions

  • Harsh DweepEmail author
  • Norbert Gretz
  • Carsten Sticht
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1182)

Abstract

miRWalk (http://mirwalk.uni-hd.de/) is a publicly available comprehensive resource, hosting the predicted as well as the experimentally validated microRNA (miRNA)–target interaction pairs. This database allows obtaining the possible miRNA-binding site predictions within the complete sequence of all known genes of three genomes (human, mouse, and rat). Moreover, it also integrates many novel features such as a comparative platform of miRNA-binding sites resulting from ten different prediction datasets, a holistic view of genetic networks of miRNA–gene pathway, and miRNA–gene–Online Mendelian Inheritance in Man disorder interactions, and unique experimentally validated information (e.g., cell lines, diseases, miRNA processing proteins). In this chapter, we describe a schematic workflow on how one can access the stored information from miRWalk and subsequently summarize its applications.

Key words

miRWalk MicroRNA Promoter Prediction 5′-UTR CDS 3′-UTR Pathways OMIM Validated Application 

Notes

Acknowledgements

This work is funded by the Research Council through Graduiertenkolleg 886 and by the German Federal Ministry of Research and Education through the National Genome Research Network (NGFN-2, Grant no. 01GR 0450).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Medical Faculty Mannheim, Medical Research CenterUniversity of HeidelbergMannheimGermany

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