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Eosinophils pp 13-20 | Cite as

Eosinophil Purification from Peripheral Blood

  • Praveen AkuthotaEmail author
  • Kelsey Capron
  • Peter F. Weller
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Eosinophils are granulocytes integral to allergic inflammation and parasitic responses and comprise 1–4 % of the circulating leukocytes in human beings under normal conditions. Isolation of human eosinophils allows for ex vivo and in vitro experimentation, providing a valuable tool for the study of allergic mechanisms. Here, we describe a technique for the isolation of human eosinophils by negative selection from whole blood obtained by venipuncture.

Key words

Eosinophils Cell separation/instrumentation Cell separation/methods Granulocytes Humans 

Notes

Acknowledgments

This work was supported by a HOPE Pilot Grant from the American Partnership for Eosinophilic Disorders (to PA) and National Institutes of Health grants R01AI051645 and R37AI020241 (to PFW).

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Praveen Akuthota
    • 1
    • 2
    Email author
  • Kelsey Capron
    • 2
  • Peter F. Weller
    • 2
    • 3
  1. 1.Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonUSA
  2. 2.Division of Allergy & Inflammation, Department of Medicine, Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonUSA
  3. 3.Division of Infectious Diseases, Department of Medicine, Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonUSA

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