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Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images

  • David P. LivingstonIIIEmail author
  • Tan D. Tuong
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1166)

Abstract

Histological analysis of frozen and thawed plants has been conducted for many years but the observation of individual sections provides only a 2-dimensional representation of a 3-dimensional phenomenon. Most techniques for viewing internal plant structure in three dimensions are either low in resolution or the instrument cannot penetrate deep enough into the tissue to visualize the whole plant. Techniques with higher resolution are expensive and equipment often requires time-consuming training. We present a relatively simple and less-expensive technique using pixel-based (JPEG) images of histological sections of an Arabidopsis thaliana plant and commercially available software to generate 3D reconstructions of internal structures. The technique has proven to work just as effectively for images from medical histology.

Key words

3D reconstruction Arabidopsis thaliana Adobe After Effects Freezing tolerance Histology Safranin Microscopy Color keying 

Supplementary material

(MP4 10,792 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.USDA-ARS and North Carolina State UniversityRaleighUSA

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