Axon Growth and Regeneration pp 167-175

Part of the Methods in Molecular Biology book series (MIMB, volume 1162)

In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regeneration

Protocol

Abstract

Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.

Key words

In vivo electroporation Peripheral nervous system Axon regeneration Dorsal root ganglion Adult mouse sensory neurons 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Orthopaedic SurgeryThe Johns Hopkins UniversityBaltimoreUSA
  2. 2.The Solomon H. Snyder Department of NeuroscienceThe Johns Hopkins UniversityBaltimoreUSA

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