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Axon Growth and Regeneration

Volume 1162 of the series Methods in Molecular Biology pp 167-175

Date:

In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regeneration

  • SaijilafuAffiliated withDepartment of Orthopaedic Surgery, The Johns Hopkins University
  • , Bo-Yin ZhangAffiliated withDepartment of Orthopaedic Surgery, The Johns Hopkins University
  • , Feng-Quan ZhouAffiliated withDepartment of Orthopaedic Surgery, The Johns Hopkins UniversityThe Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University Email author 

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Abstract

Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.

Key words

In vivo electroporation Peripheral nervous system Axon regeneration Dorsal root ganglion Adult mouse sensory neurons