Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants

  • Narciso Campos
  • Montserrat Arró
  • Albert Ferrer
  • Albert Boronat
Part of the Methods in Molecular Biology book series (MIMB, volume 1153)


The enzyme 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase catalyzes the NADPH-mediated reductive deacylation of HMG-CoA to mevalonic acid, which is the first committed step of the mevalonate pathway for isoprenoid biosynthesis. In agreement with its key regulatory role in the pathway, plant HMG-CoA reductase is modulated by many diverse external stimuli and endogenous factors and can be detected to variable levels in every plant tissue. A fine determination of HMG-CoA reductase activity levels is required to understand its contribution to plant development and adaptation to changing environmental conditions. Here, we report a procedure to reliably determine HMG-CoA reductase activity in plants. The method includes the sample collection and homogenization strategies as well as the specific activity determination based on a classical radiochemical assay.


3-Hydroxy-3-methylglutaryl CoA reductase HMG-CoA reductase HMGR MVA Mevalonate pathway Isoprenoid Terpenoid 



This work was supported by grants of the Spanish Ministerio de Economía y Competitividad and the Spanish Ministerio de Ciencia e Innovación (BFU2011-24208 to N.C., BIO2009-06984 to A.F. and M.A., and BIO2009-09523 to A.B., including FEDER funds), the Spanish Consolider-Ingenio Program (CSD2007-00036 Centre for Research in Agrigenomics), and the Generalitat de Catalunya (2009SGR0026).


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Narciso Campos
    • 1
    • 2
  • Montserrat Arró
    • 1
    • 3
  • Albert Ferrer
    • 1
    • 3
  • Albert Boronat
    • 1
    • 2
  1. 1.Center for Research in Agricultural Genomics (CRAG)CSIC-IRTA-UAB-UBBarcelonaSpain
  2. 2.Department of Biochemistry and Molecular Biology, Faculty of PharmacyUniversity of BarcelonaBarcelonaSpain
  3. 3.Faculty of Pharmacy, Department of Biochemistry and Molecular BiologyUniversity of BarcelonaBarcelonaSpain

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