Crop Breeding pp 75-86

Part of the Methods in Molecular Biology book series (MIMB, volume 1145)

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SNP Genotyping: The KASP Assay

Protocol

Abstract

The KASP genotyping assay utilizes a unique form of competitive allele-specific PCR combined with a novel, homogeneous, fluorescence-based reporting system for the identification and measurement of genetic variation occurring at the nucleotide level to detect single nucleotide polymorphisms (SNPs) or inserts and deletions (InDels). The KASP technology is suitable for use on a variety of equipment platforms and provides flexibility in terms of the number of SNPs and the number of samples able to be analyzed. The KASP chemistry functions equally well in 96-, 384-, and 1,536-well microtiter plate formats and has been utilized over many years in large and small laboratories by users across the fields of human, animal, and plant genetics.

Key words

SNPs Genotyping platform KASP High throughput Genetic variation 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Generation Challenge ProgrammeCIMMYTEstado de MéxicoMexico
  2. 2.LGC Genomics LtdHoddesdonUK

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