Crop Breeding pp 203-211 | Cite as

Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.)

  • Ainur Ismagul
  • Iryna Mazonka
  • Corinne Callegari
  • Serik Eliby
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1145)

Abstract

Barley biotechnology requires efficient genetic engineering tools for producing transgenic plants necessary for conducting reverse genetics analyses in breeding and functional genomics research. Agrobacterium-mediated genetic transformation is an important technique for producing barley transgenics with simple low-copy number transgenes. This chapter reports a refined protocol for the systematic high-throughput transformation of the advanced Australian spring barley breeding line WI4330.

Key words

Agrobacterium Barley Biotechnology Cereals Transgenic 

References

  1. 1.
    Goedeke S, Hensel G, Kapusi E et al (2007) Transgenic barley in fundamental research and biotechnology. Trans Plant J 1:104–117Google Scholar
  2. 2.
    Fell J, Miller M, Brown A et al (2011) Australian crop report—Australian Natural Resources Data Library. No. 160, ABARES, Canberra. http://adl.brs.gov.au/data/warehouse/aucrpd9abcc003/aucrpd9abcc003201112/ACR11.4_Dec_rev1.0.0.pdf. Accessed 16 Oct 2012
  3. 3.
    Wan Y, Lemaux PG (1994) Generation of large numbers of independently transformed fertile barley plants. Plant Physiol 104:37–48PubMedCentralPubMedGoogle Scholar
  4. 4.
    Tingay S, McElroy D, Kalla R et al (1997) Agrobacterium tumefaciens-mediated barley transformation. Plant J 11:1369–1376CrossRefGoogle Scholar
  5. 5.
    Bartlett JG, Alves SC, Smedley M et al (2008) High-throughput Agrobacterium-mediated barley transformation. Plant Methods 4:1–22CrossRefGoogle Scholar
  6. 6.
    Hensel G, Kastner C, Oleszczuk S et al (2009) Agrobacterium-mediated gene transfer to cereal crop plants: current protocols for barley, wheat, triticale, and maize. Int J Plant Genomics. ID 835608:9Google Scholar
  7. 7.
    Harwood WA (2012) Advances and remaining challenges in the transformation of barley and wheat. J Exp Bot 63:1791–1798PubMedCrossRefGoogle Scholar
  8. 8.
    Li M, Singh R, Bazanova N et al (2008) Spatial and temporal expression of endosperm transfer cell-specific promoters in transgenic rice and barley. Plant Biotechnol J 6:465–476PubMedCrossRefGoogle Scholar
  9. 9.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Ainur Ismagul
    • 1
    • 2
  • Iryna Mazonka
    • 1
    • 2
  • Corinne Callegari
    • 1
    • 2
  • Serik Eliby
    • 1
    • 2
  1. 1.Australian Centre for Plant Functional GenomicsUniversity of AdelaideGlen OsmondAustralia
  2. 2.School of Agriculture, Food and Wine, Waite Research Institute, University of AdelaideUrrbraeAustralia

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