Crop Breeding pp 155-165 | Cite as

TILLING for Plant Breeding

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1145)

Abstract

TILLING is widely used in plant functional genomics. Mutagenesis and SNP detection is combined to allow for the isolation of mutations in genes of interest. It can also be used as a plant breeding tool, whereby variation in known or candidate genes of interest to breeding programs is generated. Here we describe a simple low-cost TILLING procedure.

Key words

CEL 1 Mismatch Mutation SNP 
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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Plant Breeding Institute, University of SydneyNarellanAustralia

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